Diafiltration (Buffer Exchange) Using Hollow Fiber Membranes instead of Dialysis Tubing - Automated Diafiltration

Diafiltration is term used reflecting tangential flow filtration (TFF) method of “washing” or removing a permeable molecule (impurities, salts, solvents, small proteins, etc) from a solution in an accelerated method when compared to dialysis tubing. By selecting the appropriate membrane(s), it can also be used to exchange buffers, narrow particle size distributions, modify salt concentrations or maximize protein recovery in a clarification process. Buffer exchange, the process of using an ultrafiltration membrane to rapidly and gently replace one buffer of a protein solution with another, is one of the most common applications of diafiltration with Spectrum Laboratories, Inc. hollow fiber modules.